标题：Relative Importance of Phytohemagglutinin (Lectin) and Trypsin-Chymotrypsin Inhibitor on Bean (Phaseolus vulgaris L) Protein Absorption and Utilization by the Rat
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1998
卷号：44
期号：5
页码：685-696
DOI：10.3177/jnsv.44.685
出版社：Center for Academic Publications Japan
摘要：The main objective of this work was to perform a com-parative study of the antinutritional and/or toxic properties of phyto-hemagglutinin and trypsin-chymotrypsin inhibitor extracted from the seed of a commercial cultivar of edible bean used in Brazil. Bean pro-teins were extracted in acidic salt solution and fractionated by dialysis and centrifugation, then freeze-dried. The total freeze-dried bean extract and the globulin or albumin protein fraction were resuspended in distilled water and heated (100°C, 30 min) for inactivation of hemagglutinin. Diets were prepared with unheated bean protein fractions and heated ones (100% trypsin inhibitor activity, but 0 % phytohemagglutinin activity). As a result, the inhibition of growth and poor dietary protein utilization were observed in rats fed diets containing unheated bean protein fractions, but not in rats fed diets containing heated fractions. It was thus assumed that phytohemagglutinin is the main antinutritional and toxic factor in dry bean ( Phaseolus ) protein and that trypsin inhibitor (Bowman-Birk type) did not interfere with rat growth.
