期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1998
卷号：44
期号：1
页码：165-176
DOI：10.3177/jnsv.44.165
出版社：Center for Academic Publications Japan
摘要：Ethanol in the presence of disulfiram (N, N, N', N'-tetra-ethylthiuram disulfide, an inhibitor of aldehyde dehydrogenase) inhib-ited liver β-alanine-oxoglutarate aminotransferase (β-AlaAT I) activity yet activated tyrosine aminotransferase (TAT) in weanlirrg rats in vivo. The effect on fi-AIaAT I was followed by the inhibitory expression of β-AIaAT I mRNA. The β-AIaAT I activity was reduced with a pseudo-first-order profile with time, and the half-life was calculated to be 12.3±0.83h with the rate constant ( K d) of 0.056±0.004h-1. The synthesis of β-AIaAT Tin rat liver was estimated to be 1.56×10-10 mol/g of wet tissue per hour at a steady state. A combination of ethanol and disulfiram also reduced β-alanine-pyruvate aminotransferase (β-AIaAT II) activity to 60% of the control after 24h.
