期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1996
卷号：42
期号：5
页码：377-386
DOI：10.3177/jnsv.42.377
出版社：Center for Academic Publications Japan
摘要：The nutritional effects of pyridoxine-a-glucoside (PN-α-Glc), pyridoxine-5'β-glucoside (PN-5'β-Glc) and ε-N-(phosphopyridoxyl)-lysine (PNP-Lys) were examined by means of: 1) transport across the intestinal wall using everted rat intestine, 2) metabolic conversion by liver or kidney homogenate, and 3) oral administration of the compound to B6-deficient rats and the subsequent analysis of B6 derivatives found in plasma. Using everted sacs prepared from rat small intestine, PN-α-Glc was transported into the serosal side in its intact form. On the other hand, a part of PN-5'β-Glc was found as PN on the serosal side (PN-5'β-Glc: PN=2:1) . When PN-α-Glc, PN-5'β-Glc or PNP-Lys was incubated with liver or kidney homogenate for 3h at pH 6.0, PN-α-Glc was hydrolyzed to PN (6%), while there was no hydrolysis of PN-5'β-Glc. After 30min of administration of each B6 derivative to B6-deficient rats, blood was collected from the heart, and the B6-derivatives found in plasma were analyzed. It was ascertained that PN-α-Glc served as well as PN as a B6 nutrient, while PN-5'β-Glc and PNP-Lys were not easily metabolized to the coenzyme form, pyridoxal 5'-phosphate.
关键词：bioavailability of vitamin B6 derivatives;pyridoxine glucosides;ε-N-(phosphopyridoxyl)lysine;transport of vitamin B6 derivatives by everted intestinal sac
