期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2002
卷号：48
期号：6
页码：443-447
DOI：10.3177/jnsv.48.443
出版社：Center for Academic Publications Japan
摘要：The intravenous administration of β-cryptoxanthin (320μg/mouse) into 4-wk-old ddY mice caused a new peak in addition to the peak of β-cryptoxanthin during highperformance liquid chromatography (HPLC) of the lipid fraction of lung homogenate. Different HPLC conditions revealed that the new peak might be attributed to a β-carotenelike compound. The average retention times for the new peak and authentic all- trans -β-carotene were 14.97 and 15.00min, respectively, in a HPLC system using a YMC-Pack ODS-A column and methanol-based mobile phase, and 27.05 and 26.93min, respectively, in a HPLC system using a Waters Nova Pack C18 column and methanol-based mobile phase. In a HPLC system using a Waters Nova Pack C18 column and acetonitrile-based mobile phase, the retention times were 10.73, 10.48 and 10.70min for the new peak, authentic all- trans -β-carotene, and 9- cis -β-carotene, respectively. Spectrophotometry with a photodiode array detector showed maximum absorption of 447 and 475nm for the new peak, and 450 and 475nm for authentic all- trans -β-carotene. This new peak was not observed in the lung tissue of control mice. These findings indicate the possible conversion of β-cryptoxanthin to a β-carotene like-compound in ddY mice.
关键词：β-cryptoxanthin metabolism;β-carotene formation;reductive metabolism of carotenoids;mouse lung
