期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1992
卷号：38
期号：3
页码：287-296
DOI：10.3177/jnsv.38.287
出版社：Center for Academic Publications Japan
摘要：Pectin, guar gum, transgalactosylated oligosaccharide and glucose were estimated as substrate for hindgut fermentation by meas-uring the volume of gas released from a 1ml scale batch culture using rat cecal contents. The amount of short-chain fatty acids (SCFA) in cultures of glucose and pectin was also measured. The amount of SCFA ( Y μmol/ culture) or volume of gas released (Y ml/culture) was expressed as an exponential function of incubation time ( t ) ( Y = A + B × (1-e- kt ); A , B and k are constants). The amount of potential production of gas and SCFA ( B ) for glucose was higher than that for pectin and the rate constant of gas and SCFA ( k ) for pectin was higher than that for glucose. The volume of gas released (ml/culture) correlated positively and linearly with the amount of total SCFA (acetic, propionic and n -butyric acids, μmol/culture). A single regression stood for both glucose and pectin. The gas release followed saturation kinetics. The half maximal concen-tration and maximal velocity for gas release from pectin were higher than those from other substrates (glucose, guar gum and transgalactosylated oligosaccharide).
