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标题：Protein-Binding Properties of 22-Oxa-1α, 25-Dihydroxyvitamin D3, a Synthetic Analogue of 1α, 25-Dihydroxyvitamin D3
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作者：Toshio OKANO ; Naoko TSUGAWA ; Sonoko MASUDA 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1989
卷号：35
期号：5
页码：529-533
DOI：10.3177/jnsv.35.529
出版社：Center for Academic Publications Japan
摘要：Protein binding properties of 22-oxa-lα, 25-dihydroxyvitamin D₃(22-oxa-1, 25-D₃), a synthetic analogue of 1α, 25-dihydroxyvitamin D₃(1, 25-D₃), were compared with those of vitamin D₃derivatives. The order of binding affinity to the chick embryonic intestinal receptor was 1, 25-D₃>22-oxa-1, 25-D₃>25-hydroxyvitamin D₃(25-D₃) >24R, 25-dihydroxyvitamin D₃(24, 25-D₃) >vitamin D₃(D₃), while that to the rat plasma vitamin D-binding protein (DBP) was 25-D₃>24, 25-D₃>D₃>1, 25-D₃>22-oxa-1, 25-D₃. The binding potencies of 22-oxa-1, 25-D₃to the receptor and DBP were about 1/8 and 1/600 of the respective values of 1, 25-D₃. When the distribution of the tritiated compounds in human plasma components was examined by an in vitro polyacrylamide gel electrophoretic method, [³H]-22-oxa-1, 25-D₃was found to bind only to the lipoproteins including chyromicron. These results suggest that the replacement of a carbon atom into an oxygen atom in the side chain structure of 1, 25-D₃results significant decrease in the binding affinity to DBP and that 22-oxa-1, 25-D₃is transported as a complex-form not with DBP but with lipoprotein to the target tissues.
关键词：vitamin D3;1α, 25-dihydroxyvitamin D3;22-oxa-1α;25-dihydroxyvitamin D3;binding property