期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1989
卷号：35
期号：4
页码：303-313
DOI：10.3177/jnsv.35.303
出版社：Center for Academic Publications Japan
摘要：The effects of dietary pantethine levels on the drugmetabolizing system were investigated under administration of varying amounts of autoxidized linoleate (AL) with rat liver microsomes and S-9 fractions. AL having 800meq/kg of peroxide value and 1, 700meq/kg of carbonyl value was dosed to the rats of each group given drinking water containing 0mg% (deficient), 6.25mg% (normal), and 125mg% pantethine (sufficient). The contents and activities of the enzymes in the drugmetabolizing system in the rat liver of each pantethine-level group changed essentially in a similar manner, that is, they were induced at an AL daily dose of 0.2ml/100g body weight ( i.e. , small dose) for 5 successive days and lowered at a daily dose of 0.4ml/100g body weight ( i.e. , large dose) by the same administration period, compared with respective nonAL groups in each of the three pantethine levels. In both non-AL and the small-dose AL, enzyme activities of the electron transfer system in rat liver microsomes, aminopyrin-N-demethylase activity, and metabolic activation of 2-acetylaminofluorene in S-9 fractions were significantly higher in the pantethine-deficient group than in the pantethine-normal and sufficient groups. In the large-dose AL, the enzyme activities in the drugmetabolizing system decreased significantly in any pantethine levels, though the survival rate of the rats was higher in the pantethine-sufficient group than in the pantethine-normal groups. The results suggest that the pantethine relieves the effect of dosed AL on the drug-metabolizing system in rat liver.
关键词：autoxidized linoleic acid;microsome;cytochrome P-450;cytochrome b5;electron transfer system;drug-metabolizing activity;aminopyrin-N-demethylase;S-9 activity;pantethine
