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  • 标题:Synthesis and Assignment of Novel [125I]-Labeled 1α, 25-Dihydroxyvitamin D3 Derivatives
  • 本地全文:下载
  • 作者:Miyuki TANABE ; Masaya HARADA ; Fumitomo ODAWARA
  • 期刊名称:Journal of Nutritional Science and Vitaminology
  • 印刷版ISSN:0301-4800
  • 电子版ISSN:1881-7742
  • 出版年度:1991
  • 卷号:37
  • 期号:2
  • 页码:139-147
  • DOI:10.3177/jnsv.37.139
  • 出版社:Center for Academic Publications Japan
  • 摘要:This paper describes the synthesis and structural assignment of novel 125I-l α, 25-dihydroxyvitamin D3 derivatives (1a) and (lb) labeled with 125I-Bolton-Hunter reagent [ N -succinimidyl 3-(4-hydroxy-3-iodo [125I] phenyl)propionate] ( 1 ), which is known as a protein-labeling reagent, as tracers for radioimmunoassay (RIA). The radiospecific activities of these tracers (1a) and (lb) were calculated as 2, 200 Ci/mmol (81.4TBq/mmol).
  • 关键词:1α, 25-dihydroxyvitamin D3;iodo[125I]-labeled 1α;25-dihydroxyvitamin D3;125I-Bolton-Hunter reagent;anti-1α;25-dihydroxyvitamin D3 sheep serum;radiommunoassay (RIA)
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