期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1990
卷号：36
期号：6
页码：521-529
DOI：10.3177/jnsv.36.521
出版社：Center for Academic Publications Japan
摘要：To investigate the validity of using HPLC procedure with the fluorescence detection for the estimation of the pyridoxal 5'-phosphate (PLP) concentration in human blood plasma, we modified the conditions of our previous HPLC method for PLP. By changing the pH of the mobile phase (pH 5.5) and by altering the detection wavelengths in fluorescence (excitation at 322nm and emission at 417nm), both the improved sensitivity for pyridoxic acid 5'-phosphate and elimination of interfering extraneous peaks were attained. We reported the total vitamin B6 content of healthy adult volunteers obtained by this HPLC method.
关键词：HPLC analysis of pyridoxal 5'-phosphate;pyridoxal 5'-phosphate contents in human plasma;pyridoxic acid 5'-phosphate;KCN treatment of pyridoxal 5'-phosphate;vitamin B6 analysis in human plasma by HPLC
