期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1977
卷号：23
期号：6
页码：525-534
DOI：10.3177/jnsv.23.525
出版社：Center for Academic Publications Japan
摘要：A lethal protein with hemagglutinating activity but with-out trypsin inhibitory activity was isolated from beans of Phaseolus vulgaris, cultiva , and Kintoki and proved homogeneous by ultracentri-fugation, disc polyacrylamide gel electrophoresis, sodium dodesyl sulfate polyacrylamide gel electrophoresis and isoelectric focusing. The mole cular weight was estimated to be 104, 000 by ultracentrifugal analysis and gel filtration on Sephadex G-200. The molecule dissociates into three identical subunits in the presence of 8M urea or 0.1% sodium dodesyl sulfate. The amino acid composition was characterized by the high content of aspartic acid and the complete absence of methionine and cystine. The carbohydrate content was 8.1%; 5.0% mannose and 3.1% glucosamine. The addition of the lethal protein to a basal diet (0.4%) resulted in the intensive depression of the growth and finally in the death of rats. The intraperitoneal injection of 250μg per g body weight of mouse brought about an acute toxicity which caused death of all the injected mice.
