期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1977
卷号：23
期号：4
页码：291-298
DOI：10.3177/jnsv.23.291
出版社：Center for Academic Publications Japan
摘要：An improved procedure for the isolation of suprasterol2 I and II from a photochemical reaction mixture of ergocalciferol (vitamin D2) and their spectral data are described in this paper, when a solution of ergocalciferol in ethanol was irradiated by UV light from a high-pressure mercury lamp, the reaction mixture gave six spots, including suprasterol2 I and II, on the thin-layer chromatogram, while the peaks corresponding to pyro-D2, isopyro-D2, 5, 6- trrans -D2, suprasterol2 I and II were observed in the gas chromatogram obtained from a capillary column GLC (Supra-sterol2 I and II were main peaks). After purifying the mixture by column chromatography on silica gel containing 12% alumina as an adsorbent, two main fractions were isolated. The data of their spectra, TLC and GLC showed that the former fraction was suprasterol2 II while the latter was suprasterol2 I and that the both fractions contained the respective compound only. Both suprasterol2 were crystallized as the 3, 5-dinitro-benzoates.
