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  • 标题:THE INTRACELLULAR LOCALIZATION AND STABILITY OF THE DIHYDROFOLATE SYNTHETASE IN PEA SEEDLINGS
  • 本地全文:下载
  • 作者:Masamichi IKEDA ; Kazuo IWAI
  • 期刊名称:Journal of Nutritional Science and Vitaminology
  • 印刷版ISSN:0301-4800
  • 电子版ISSN:1881-7742
  • 出版年度:1975
  • 卷号:21
  • 期号:1
  • 页码:1-6
  • DOI:10.3177/jnsv.21.1
  • 出版社:Center for Academic Publications Japan
  • 摘要:The amount of folate compounds rapidly increased during germination of the pea. An especially high increase was observed 2 days after sowing, with a continuously increasing content during germination. Dihydrofolate synthetase [EC 6.3.2.12] activity also increased with the in-crease in the amount of folate compounds, and the maximum activity was observed at 6 days after sowing in the dark and at 8 days after sowing in the light. The dhydrofolate synthetase was localized mostly in the mitochondrial fraction and it was easily extracted from the cell particles by osmotic shock. A similar localization was also observed in spinach leaves. The enzyme which was extracted from the isolated mitochondria was relatively stable in comparison with that extracted from whole cells.
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