期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1988
卷号：34
期号：1
页码：1-10
DOI：10.3177/jnsv.34.1
出版社：Center for Academic Publications Japan
摘要：The activity of an enzyme involved in decyanation of cyanocobalamin was found in the cell homogenate of Euglena gracilis . The enzyme essentially required FAD or FMN, and NADPH as cofactors. The apparent K m for cyanocobalamin and NADPH were 7.1μM and 0.2 mM, respectively. The enzyme reaction obeyed allosteric kinetics towards FAD ([FAD]0.5=30μM, n=2.7; as calculated by the Hill plots). The Euglena enzyme was located in the mitochondria.
