期刊名称:Journal of Nutritional Science and Vitaminology
印刷版ISSN:0301-4800
电子版ISSN:1881-7742
出版年度:1988
卷号:34
期号:1
页码:97-106
DOI:10.3177/jnsv.34.97
出版社:Center for Academic Publications Japan
摘要:Autoxidized linoleic acid (AL) having 800meq/kg of per-oxide value and 1, 700 meq/kg of carbonyl value was given in repeated oral doses at a daily dose of 0 (control)-7.5ml/kg to male Wistar rats for 5 successive days. The effect of increasing AL dose on the drug-metabolizing system was investigated in rat liver microsomes and S-9 fractions. All the rats of a daily dose of 5.0-7.5ml/kg died after the third day of consecutive oral doses. The cytochrome P-450 and b 5 contents, enzyme activities in electron transfer system, aminopyrin-N-demethylase activity and S-9 activity (metabolic activation of 2-acetylaminofluorene) in the drug-metabolizing system changed essentially in a similar manner, that is, both the contents and the activities were increased by a small dose of AL, and were decreased by a large dose of AL. These results strongly supported the findings in a previous report wherein we observed the periodical effect of AL dose on the drug-metabolizing system.
关键词:autoxidized linoleic acid;microsome;cytochrome P-450;cytochrome b5;electron transfer system;drug-metabolizing activity;aminopyrin-N-demethylase;S-9 activity;membrane disorder