期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1983
卷号：29
期号：6
页码：671-678
DOI：10.3177/jnsv.29.671
出版社：Center for Academic Publications Japan
摘要：Lambda DNA-fragmenting actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ were studied. The mixture of AsA or TR and Cu2+ caused a marked fragmentation ofλ, DNA (3.2×107 daltons) within the first 1 min of reaction. Further incubation resulted in accumulation of the most abundant species of fragmented λDNA having a molecular weight of 1.3×105 daltons. The mixture of AsA or TR and Cu2+ fragmented calf thymus DNA to produce the fragmented DNA of which 5'-OH terminal groups have a mixture of free OH groups and phosphodiester linkage. The mixture of AsA or TR and Cu2+ was also found to fragment, DNA to produce dCMP predominantly as 5'-OH terminal nucleotides.
关键词：λDNA-fragmenting action;ascorbic acid;triose reductone;λDNA;Cu2+;agarose slab gel electrophoresis;5'-OH terminal nucleotides
