期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1983
卷号：29
期号：5
页码：533-544
DOI：10.3177/jnsv.29.533
出版社：Center for Academic Publications Japan
摘要：The effect of pyridoxal 5'-phosphate on the 1, 25-dihydroxyvitamin D3 receptor system has been studied by using pig intestinal chromatin. Pyridoxal 5'-phosphate did not affect the binding of 1, 25-dihydroxyvitamin D3 to its receptor extracted from chromatin with hypertonic KCI, although in the presence of pyridoxal 5'-phosphate 1, 25-dihydroxyvitamin D3-receptor complexes were not readily precipitated with polyethylene glycol. In contrast, pyridoxal 5'-phosphate showed a potency to dissociate the 1, 25-dihydroxyvitamin D3 receptor from chromatin in a dose-dependent manner. A low concentration of pyridoxal 5'phosphate was as effective as hypertonic KCl in dissociating the receptor from chromatin, while pyridoxine, p -nitrophenyl phosphate, or inorganic phosphate was much less effective. These observations suggest the inhibitory effect of pyridoxal 5'-phosphate on the recognition of 1, 25-dihydroxyvitamin D3 by its receptor system.
