期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1981
卷号：27
期号：1
页码：1-7
DOI：10.3177/jnsv.27.1
出版社：Center for Academic Publications Japan
摘要：High-performance liquid chromatography (HPLC) was applied to the determination of ascorbic acid (AsA) and erythorbic acid (ErA). The apparatus was a Shimadzu model LC-2P Liquid Chromatograph equipped with a UV detector set at 254nm. The separation was achieved on a LiChrosorb-NH2 column which was pre-treated with 0.1M ammonium monophosphate solution using a mixture of acetonitrile, acetic acid and water (87:2:11, v/v) as an eluant. The HPLC method has the following advantages: AsA and ErA are quantitated after being distinctly separated, analysis time per one sample is short, and AsA or ErA levels as low as 1.0×10-2μg are detectable. Recovery experiments with dehydro-AsA and dehydro-ErA, involving reduction with H2S, gave satisfactory results.
