期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1980
卷号：26
期号：6
页码：535-543
DOI：10.3177/jnsv.26.535
出版社：Center for Academic Publications Japan
摘要：4'-Substituted pyridoxine analogues (4'-PN analogues), which were potent antagonists of vitamin B6, induced severe convulsions in mouse which could be prevented by vitamin B6. To elucidate the mechanism of the convulsive action, the effect of 4'-PN analogues on partially purified pyridoxal kinase (PL-kinase) from mouse brain was studied. 4'-Methoxypyridoxine (4'-MPN), 4'-ethoxypyridoxine (4'-EPN), 4'-propoxypyridoxine (4'-PPN) and 4'-isopropoxypyridoxine (4'-iso PPN) were found to be powerful inhibitors of PL-kinase and competed with pyridoxal (PL). There was a good relationship between mouse convulsions and inhibitory activities of PL-kinase. We also studied whether 4'-MPN, 4'-EPN, 4'-PPN and 4'-isoPPN were used as substrates of PL-kinase. High-performance liquid chromatography was used to separate 4'-PN 5'-phosphate analogues and ADP which were formed in the enzyme reaction, and to measure them quantitatively. The results showed that 4'-MPN, 4'-EPN, 4'-PPN and 4'-isoPPN were also phosphorylated by PL-kinase like 4'-deoxypyridoxine.
关键词：vitamin B6;competitive inhibition of PL-kinase;high-perfor mance liquid chromatography;4'-deoxypyridoxine;4'-methoxypyri doxine;4'-ethoxypyridoxine;4'-propoxypyridoxine;4'-isopropoxypyri doxine
