期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1980
卷号：26
期号：1
页码：87-97
DOI：10.3177/jnsv.26.87
出版社：Center for Academic Publications Japan
摘要：Two-site immunoradiometric assay for serum ferritin was developed by anti-human liver ferritin antiserum applied on polyvinyl V bottom microtiter plates. The coefficient of variation was 4.45.9% of liver ferritin dissolved in 1/4 diluted human serum at a concentration of 0.625-125ng/ml. The lowest concentration (0.625ng/ml) was statistically distinguishable from the background by Student's t test ( p <0.05). Isoferritin patterns of serum ferritin and tissue ferritins were examined by the combination of gel isoelectric focussing and 2-site immunoradiometric assay in order to explore any similarities in pls within these ferritins. Although serum ferritin had wide range of pls, its basic isomers corresponded well to those of liver and spleen ferritin. Geometric means and their 95% confidence limits in serum ferritin concentration were 70ng/ml and 12-411ng/ml, respectively, in sera of 205 healthy males (16-25 years), and 12 ng/ml and 1-211ng/ml, respectively, in 421 healthy females (16-59 years).
