期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1976
卷号：22
期号：1
页码：15-20
DOI：10.3177/jnsv.22.15
出版社：Center for Academic Publications Japan
摘要：The simultaneous determination of vitamin D2 and its isomers by high-speed liquid chromatography (HSLC) is described. Preferred operating conditions for the resolution and quantitation of vitamin D2, 5, 6- traps -vitamin D2, ergosterol, lumisterol2, isovitamin D2, isotachysterol2, tachysterol2, and previtamin D2 are as follows: column, “Zorbax” SIL (25cm×2.1mm i.d. ); pressure, 100-120kg/cm2; temperature, ambient; detector, UV 254nm; mobile phase, 0.15% methanol+2% ether in pentane (for the simultaneous determination), 10% ether in hexane (for quantitation of the practical sets of isomers), or 55% CHCl3 (distilled) in pentane (for separation of isovitamin D2 and 5, 6-traps-vitamin D2); sample size, 1 μl (>1-5 ng); internal standard, p -cresol or α-naphthol. High-speed, good resolution, precise and accurate trace analysis, greater analysis flexibility, no necessity for preparing derivatives, and gentle operating conditions are main potential advantages of our method which has proved to be very efficient and surpasses all analytical procedures hitherto proposed.
