期刊名称:Journal of Nutritional Science and Vitaminology
印刷版ISSN:0301-4800
电子版ISSN:1881-7742
出版年度:1975
卷号:21
期号:6
页码:421-428
DOI:10.3177/jnsv.21.421
出版社:Center for Academic Publications Japan
摘要:A trypsin inhibitor was extracted from eggplant exocarps with several buffers. The 0.1M acetate buffer, pH 5.5, extract had the highest specific activity. The crude inhibitor, obtained by heat treatment and salting-out from the acetate buffer extract, contained 4.5 nitrogen and 22.6% hexose. Isoelectrofocusing demonstrated that this crude inhibitor in the eggplant exocarps was composed of at least three forms, one of which differed in its isoelectric point. The form at pH 4.7 had the strongest activity. The molecular weights of these inhibitors were estimated to be between 5.000-10.000 by gel filtration.