期刊名称:Journal of Nutritional Science and Vitaminology
印刷版ISSN:0301-4800
电子版ISSN:1881-7742
出版年度:1975
卷号:21
期号:3
页码:183-188
DOI:10.3177/jnsv.21.183
出版社:Center for Academic Publications Japan
摘要:A high-speed liquid chromatographic method for the determination of α-, β-, γ- and δ-tocopherols using a spectrophotometer or a spectrofluorometer, is described. The best results were obtained using a 2.3 mm i.d. ×500mm normal phase partition column, JASCO-PACK WC-03-500 and a spectrofluorometer as a specific detector. A mixture of diisopropyl ether and n -hexane (2:98) was used as the mobile phase. A flow rate was set at 0.8ml/min. A mixture of pure α-, β-, γ- and δ-tocopherols or natural tocopherols in vegetable oils were separately determined by this method.