期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2005
卷号：51
期号：4
页码：274-277
DOI：10.3177/jnsv.51.274
出版社：Center for Academic Publications Japan
摘要：We cloned and analyzed the 5'-flanking region of the human thiamin pyrophosphokinase gene ( hTPK1 ). Truncation analysis using transiently transfected HepG2 cells revealed the minimal region required for basal activity of the hTPK1 promoter, which was encoded in a sequence between -105 and +441 relative to the transcription start site. In an electrophoretic mobility shift assay using the nuclear extracts from HepG2 cells and the synthetic oligonucleotide containing the Sp 1 site, specific DNA-protein complexes were identified. These findings indicate the importance of the Spl cis -element in regulating the hTPK1 gene expression.
