标题：Deterioration of Membrane Morphology, Phospholipids, and Cytoskeletal Protein in Rat Erythrocytes Exposed to tert-Butyl Hydroperoxide: Protection by Exogenous Glutathione Fails in Selenium Deficiency
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1988
卷号：34
期号：5
页码：491-506
DOI：10.3177/jnsv.34.491
出版社：Center for Academic Publications Japan
摘要：Here we report scanning electron microscopy, phospholipid and fatty acid composition and cytoskeleton proteins of erythrocytes from Se-adequate and Se-deficient rats, showing protection by glutathione (GSH) against tent -butyl hydroperoxide ( t -Bu00H)-induced hemolysis of erythrocytes from Se-adequate, but not from Se-deficient rats. Without exogenous GSH, erythrocytes incubated with t -BuOOH exhibited remark-able deterioration of cell membranes with diminished membrane phosphatidyl-ethanolamine, -serine, and -inositol and selective loss of a cytoskeletal protein, ankyrin 2-1. Without exogenous GSH these changes occurred in erythrocytes from both Se-deficient and Se-adequate rats. Dietary Se, through provision of glutathione peroxidase (GSH-Px) in erythrocytes as a probable scavenger of t -Bu00H, protects against hemolysis when GSH is available.
