期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1997
卷号：43
期号：4
页码：463-469
DOI：10.3177/jnsv.43.463
出版社：Center for Academic Publications Japan
摘要：Glycated cytosolic aspartate aminotransferase was detected in the liver and kidney of streptozotocin diabetic rats using a boronate affinity column for adjacent cis.hydroxyl groups and an iunoblotting technique. The enzymatic activity and amount of immunoreactive subb stance were determined in the liver, kidney, and erythrocytes of diabetic and control rats. The ratio of enzymatic activity to the amount of enzyme was lower in diabetic rat tissue than in that in the control rats. It has been suggested that there is an inactive aspartate aminotransferase molecule in the tissues of diabetic rats. We therefore suggest that the cytosolic aspartate aminotransferase was inactivated in the diabetic rat tissues by a glycation reaction, accompanied by an impairment in glucose utilization.
关键词：cytosolic aspartate aminotransferase (CAST);inactivation of cAST;glycation of cAST;diabetes;streptozotocin (stz)
