标题：Repression of Fat-Dependent Intestinal Apo A-IV mRNA Abundance by Medium Chain Triacylglycerols and Proteins, and Elevation by Carbohydrates of Fat-Dependent Apo A-IV Transport in Suckling Rat Pups
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1995
卷号：41
期号：3
页码：293-306
DOI：10.3177/jnsv.41.293
出版社：Center for Academic Publications Japan
摘要：Nutrients regulating intestinal apo A-IV synthesis and se-cretion in developing rats have not been clearly defined. We examined the effect of ingestion of fats, carbohydrates and proteins on the abundance of intestinal apo A-IV mRNA and the serum concentration of apo A-IV in 14-day-old suckling rat pups fasted overnight. In pups ingesting long-chain fatty acid-fat (soybean oil, trioleino LCT), there was a prompt elevation of the mRNA at 1.5 h after ingestion, although a graded dose of soybean oil did not result in a comparable elevation of the apo A-IV mRNA. In pups on MCT, but not trilaurin, there was a repression of the LCT-dependent elevation of the message. In pups on Intralipid (com-posed of soybean oil, lecithin and glycerol), mRNA was elevated at 6 h after ingestion. Administration of Intralipid with lactose, glucose, fruc-tose and sucrose induced a rapid elevation of mRNA together with elevation of serum apo A-IV, although administration of casein, whey proteins and soybean proteins resulted in repression of the Intralipid-dependent mRNA elevation. The message correlated weakly to the serum apo A-IV and triacylglycerols and with no correlation to intestinal fat accumulation. These results suggest that metabolic events following the ingestion of milk components modulate intestinal apo A-IV expression in developing rats, possibly through mucosal fatty acid utilization.
关键词：expression of apo A-IV;apo A-IV mRNA;rat pups;intes-tine;medium chain fatty acids;carbohydrates;proteins;triacylglycerols
