期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1995
卷号：41
期号：3
页码：307-312
DOI：10.3177/jnsv.41.307
出版社：Center for Academic Publications Japan
摘要：The conditions of immobilization of lipase on the polyethylene powder were examined and the immobilized lipase was used for effective production of α-linolenic acid from perilla oil hydrolysis. Using the polyethylene powder containing micropores in its structure enabled immobilization of lipases. Ethanol pretreatment increased the immobilization efficiency. The efficiency of immobilization of six different lipases was 67-91%. On hydrolysis of perilla oil, the activity of the immobilized lipase was nearly the same as that of free lipase. Among the enzymes used, the Candida rugosa lipase hydrolyzed perilla oil most effectively. When the immobilized C. rugosa lipase was used 10 times for perilla oil hydrolysis by the batch reaction method (1 batch, 24-h reaction at 37°C), there was little decrease in its activity. These results suggest that polyethylene-immobilized lipase can be used for the effective hydrolysis of perilla oil to produce α-linolenic acid.
