期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1987
卷号：33
期号：1
页码：37-48
DOI：10.3177/jnsv.33.37
出版社：Center for Academic Publications Japan
摘要：Autoxidized linoleic acid (AL) having 800 meq/kg of peroxide value and 1, 700 meq/kg of carbonyl value was given in repeated oral doses for 1-15 days at a daily dose of 2.5 ml/kg to male Wistar rats. During the administration, the effect of AL on drug-metabolizing activity was investigated periodically in liver microsomes. The contents of cytochrome P-450 and b 5 were increased by consecutive oral doses for 3-7 days. Thereafter, the amount of cytochrome P-450 decreased gradually, but the b 5 decreased slightly. Thus, after administration for 11-15 days, the cytochrome P-450 content was significantly lower but the cytochrome b 5 content was rather high in comparison with the control group. The aminopyrin-N demethylase activity was not reduced even after repeated oral doses of AL for 15 days. The activation of 2-acetyl amino fluorene (2AAF), which the authors termed S-9 activity, gradually decreased during administration for more than 3 days, and completely disappeared after administration for 9 days.
关键词：Key Words autoxidized linoleic acid;microsome;cytochrome P-450;cytochrome b5;drug-metabolizing activity;aminopyrin-N-demethylase;S9 activity;membrane disorder
