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  • 标题:A METHOD FOR QUANTITATION OF VITAMIN B2 BY USING AN ENZYME (PRODUCING VITAMIN B2 ALDEHYDE) FROM SCHIZOPHYLLUM COMMUNE
  • 本地全文:下载
  • 作者:Sei TACHIBANA ; Masanori OKA
  • 期刊名称:Journal of Nutritional Science and Vitaminology
  • 印刷版ISSN:0301-4800
  • 电子版ISSN:1881-7742
  • 出版年度:1980
  • 卷号:26
  • 期号:5
  • 页码:419-426
  • DOI:10.3177/jnsv.26.419
  • 出版社:Center for Academic Publications Japan
  • 摘要:A method for enzymatic determinationn of riboflavin was developed by enzymatic reduction of 2, 6-dichlorophenolindophenol using a vitamin B2-aldehyde-forming enzyme prepared from Schizophyllum commune. The enzymatic method enabled us to determine riboflavin in a mixture containing riboflavin, FMN and FAD without separation of riboflavin. Moreover, the total amount of flavins in the mixture was shown to be determined by the enzyme in the presence of phosphatase.
  • 关键词:riboflavin;vitamin B2-aldehyde;Schizophyllum commune;2, 6 dichlorophenolindophenol;determination;vitamin B2-aldehyde-forming enzyme
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