期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1979
卷号：25
期号：5
页码：399-409
DOI：10.3177/jnsv.25.399
出版社：Center for Academic Publications Japan
摘要：The interaction of 1α, 24-dihydroxyvitamin D3 with the 1α, 25-dihydroxyvitamin D3 receptor system was studied in chick intestinal mucosa. The competitive receptor binding assay indicated that 1α, 24-dihydroxy-vitamin D3 bound to a cytosol 1α, 25-dihydroxyvitamin D3 receptor with a relatively high affinity compared with other vitamin D3 analogs. The R-isomer of 1α, 24-dihydroxyvitamin D3 revealed higher affinity for the receptor than the S-isomer. In the reconstituted cytosol-chromatin system, both 1α, 24(R)-dihydroxy-[3H]-vitamin D3 and 1α, 24(S)-dihydroxy-[3H]-vitamin D3 specifically associated with chromatin via a temperature-dependent process. The association of 1α, 24-dihydroxy-[3H]-vitamin D3 with chromatin was reduced in the presence of competing unlabeled 1α, 25-dihydroxyvitamin D3. Furthermore, the chromatin-associated 1α, 24-dihydroxy-[3H]-vitamin D3 was dissociated by a high concentration of KCl, likewise 1α, 25-dihydroxy-[3H]-vitamin D3. From these results, it is strongly indicated that 1α, 24-dihydroxyvitamin D3 is recognized by cytosol 1α, 25-dihydroxyvitamin D3 receptor as an analog of 1α, 25-dihydroxyvitamin D3 and associates with chromatin by the same mechanism as 1α, 25-dihydroxyvitamin D3.
