期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1979
卷号：25
期号：2
页码：59-66
DOI：10.3177/jnsv.25.59
出版社：Center for Academic Publications Japan
摘要：Neutral lipids, especially triacylglycerols, accumulated due to myo -inositol deficiency both in the cells of Saccharomyces carlsbergensis (HAYASHI et al . (1976) J. Biol. Chem ., 251 , 5759-5769) and in the liver of the rat (HAYASHI et al . (1974) Biochim. Biophys. Acta , 360 , 134-155). The accumulation of triacylglycerols in the deficient yeast resulted, at least partly, from an enhancement of acetyl CoA carboxylase activity. The activation of the enzyme reflected the fluctuation due to the deficiency in the levels of fructose bisphosphate and citrate (HAYASHI et al . (1978) Biochim. Biophys. Acta , 540 , 231-237). Thus, the kinetics of the regulation of acetyl CoA carboxylase by these intermediates was studied. In physiological concentrations fructose bisphosphate sigmoidally activated acetyl CoA carboxylase from yeast with the Hill coefficient of 3, while citrate counteracted the fructose bisphosphate activation in a sigmoidal manner with the Hill coefficient of 2. Fructose bisphosphate markedly increased the apparent V max value of acetyl CoA carboxylase for the substrate, ATP and slightly decreased the apparent Km value. Citrate greatly decreased the apparent V max value increased by fructose bisphosphate.
关键词：acetyl CoA carboxylase;Saccharomyces carlsbergensis;citrate;fructose bisphosphate;Vmax;Km;myo-inositol deficiency;neutral lipid accumulation
