期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1978
卷号：24
期号：6
页码：555-567
DOI：10.3177/jnsv.24.555
出版社：Center for Academic Publications Japan
摘要：Two kinds of high-speed liquid chromatographic methods using either ultraviolet or a fluorometric detector (HSLC-UV and HSLC-fluorometric methods) are described for the determination of ubiquinones (UQ). HSLC-UV method: Serum or liver was saponified with methanolic alkaline solution containing pyrogallol as an antioxidant and 2, 3, 6trimethyl-5-nonaprenyl-l, 4-benzoquinone as an internal standard and then extracted with n -hexane. The extract was evaporated and then dissolved in dioxane. The resulting solution was applied to a HSLC system using a Permaphase ODS column, ethanol-water (80:20) as a mobile phase and a high sensitivity UV detector (275 nm). HSLC-fluorometric method: Serum was deproteinized with ethanol and then extracted with n -hexane. The resulting extract was reacted with alkaline ethylcyanoacetate (ECA) reagent to give fluorescence. The reacted solution was applied to a HSLC system using a Permaphase ODS column, ethanol-water (75:25) as a mobile phase and a spectrofluorometer (Ex. 430 nm, Em. 530 nm). Neither of the proposed HSLC methods were affected by other fat-soluble substances and seemed to be more simple, sensitive and specific than other conventional determination methods.
