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  • 标题:IMPROVED AND EFFECTIVE ASSAYS OF THE GLUTAMIC OXALOACETIC TRANSAMINASE BY THE COENZYME-APOENZYME SYSTEM (CAS) PRINCIPLE
  • 本地全文:下载
  • 作者:Hiroe KISHI ; Karl FOLKERS
  • 期刊名称:Journal of Nutritional Science and Vitaminology
  • 印刷版ISSN:0301-4800
  • 电子版ISSN:1881-7742
  • 出版年度:1976
  • 卷号:22
  • 期号:3
  • 页码:225-234
  • DOI:10.3177/jnsv.22.225
  • 出版社:Center for Academic Publications Japan
  • 摘要:Standardized and reliable methods have been devised to assay preparations of the glutamic oxaloacetic transaminase from human erythrocytes by the CAS principle, i.e. , the unsaturation and saturation of the coenzyme (pyridoxal 5'-phosphate) of a Coenzyme-Apoenzyme System. This dual enzymic assay is effective to detect and measure human deficiencies of vitamin B6. A preferred procedure has been developed, particularly for individuals and for limited groups, but is also applicable to large groups. A simplified procedure, which emphasizes the statistical significance of difference between large groups was also developed. Lyophilized erythrocyte hemolysates appear stable for up to 7 days for simplified transportation.
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