出版社：National Institute of Occupational Safety and Health
摘要：In order to study and compare genetic damage induced by 10 kinds of man-made mineral fibers (JFM fibers) in cells, human lung epithelial cells (A549) were exposed to JFM fibers and chrysotile for 1h, then single-cell gel electrophoresis (SCGE) assay was used to detect DNA strand breaks, DNA-DNA interstrand crosslink and the ability of DNA to repair; The results showed that all 10 JFM fibers could induce DNA strand breaks, DNA-DNA interstrand crosslinks and inhibit the ability of DNA repair. When human embryo lung (HEL) cells were exposed to JFM fibers and chrysotile for 24h respectively, the chromosomal aberration was analyzed and the results showed that chrysotile and most of JFM fibers at 5.0μg/ml induced structural chromosomal aberration, but all of these effects were lower than that of chrysotile and were different among them, suggesting that 10 types of JFM fibers had genotoxicity with different degree in vitro , but all of them were lower than that of chrysotile.
关键词：Mineral fiber;DNA damage;DNA-DNA crosslinks;DNA strand breaks;DNA repair;chromosomal aberration;Single-cell gel electrophoresis
