出版社：National Institute of Occupational Safety and Health
摘要：o -Dichlorobenzene ( o -DCB) is used as an organic solvent, as a chemical intermediate, and as a heat transfer medium. In humans, o -DCB is metabolized to 2, 3- and 3, 4-dichlorophenols, and 3, 4- and 4, 5-dichlorocatechols, and these metabolites are eliminated via the kidneys. In this study, we tried to determine the concentrations of urinary 2, 3- and 3, 4-dichlorophenols using a gas chromatograph (GC). When control urine specimens were spiked at concentrations of 10, 20 and 40mg/l, the mean recovery rates of 2, 3- and 3, 4-dichlorophenols were 98.3 to 101.9% (CV=4.0 to 4.8%) and 100.6 to 105.4% (CV=2.5 to 7.0%), respectively. Next, urine samples collected from ten male workers exposed to o -DCB were analyzed. The concentrations of urinary 2, 3- and 3, 4-dichlorophenols determined by the GC method closely agreed with those by the HPLC method, which we had developed in a previous study, and these metabolite concentrations were linearly correlated to the 8-h TWA values of o -DCB in the range of 0.1 to 2.3ppm. Consequently, the GC method can be used for biological monitoring of o -DCB, though it is necessary that the linear relation is confirmed in a higher range of exposure.
