出版社：National Institute of Occupational Safety and Health
摘要：A simple and specific method for the quantitative determination of hippuric acid and methylhippuric acids is described. Urine samples were applied to a silica gel plate (silicagel, 60 F254), and developed with chloroform-acetone-acetic acid. The spots of hippuric acid and methylhippuric acids were identified by illuminating the silica gel plate with an ultraviolet lamp, scratched out and ex-tracted with pyridine. The color was then developed with acetic anhydride and p-dimethylaminobenzaldehyde (DAB) in pyridine at 40°C for 60 min. The absor-bance at 458 nm was determined against a blank containing pyridine, acetic anydride and DAB.
