摘要：Rat serum obtained at 24 h after subcutaneous injection of carrageenin significantly suppressed chemotaxis of rat polymorphonuclear leukocytes (PMNs) in vitro. α2 Acute-phase macroglobulin (α2APM), α1 proteinase inhibitor (α1PI) and cysteine-proteinase inhibitors (CPIs) are present at high concentration in the 24-h serum and known as acute-phase reactants in rats. These acute-phase proteinase inhibitors were purified from inflamed rat serum or exudate and their effect on PMN chemotaxis was studied by Boyden's method in vitro. α2APM (4 mg/ml) significantly suppressed PMN chemotaxis while α1M was without effect, though both α2APM and α1M had a similar anti-proteinase activity. The results suggest that α2APM suppressed PMN chemotaxis through the mechanism unrelated to its anti-proteinase activity. On the other hand, α1PI (1 and 3 mg/ml) slightly but significantly suppressed PMN chemotaxis, whereas CPI-1 and CPI-2 had no inhibitory effect. These results suggest that α2APM and α1PI play a role in suppression of PMNs infiltration into the inflammatory site in the late-phase of acute inflammation.
