摘要：Single smooth muscle cells were isolated from taenia coli of guinea pig, and calcium-and caffeine-contraction was studied. The degree of contraction of the single cells by calcium under the depolarized condition was dependent on the concentration of calcium but the sensitivity of the cells to calcium was less than that of intact tissue. However, cells which were responsive to calcium were 56% of the cells examined. Subsequent application of caffeine to the cells resulted in contraction of 85% of the single cells. These single cells examined in this experiment were not stained by trypan blue. The results indicated that, during isolation, certain trigger mechanism to produce contraction by exogenous calcium was hindered in a half of the cell preparation.
