摘要：A facile detection method for alcohol dehydrogenase (ADH) in the gel after electrophoresis of tissue samples was developed. The gel plate was incubated in the presence of alcohol and nicotinamide adenine dinucleotide (NAD) and the resultant greenish fluorescent spots of NADH (reduced form of NAD) were detected under a 365-nm ultraviolet (UV) lamp. The gel plate was laid on a sheet of photographic paper and exposed to a flash of the UV light. From the photograph, the activity of ADH could be estimated. Aldehyde dehydrogenase (ALDH) was similary detectable with acetaldehyde as the substrate instead of alcohol.
