摘要：A method for the determination of mercaptobenzothiazole-type accelerators (MBTs) was studied by reversed-phase high performance liquid chromatography (HPLC) with Nucleosil 5 C 18 column by using methanol-water (95 : 5) as a mobile phase. After extraction of rubber samples by shaking with an acetone-chloroform (1 : 1) mixture at room temperature, MBTs were analyzed by HPLC with ultraviolet (UV) detector at 275 nm before and after methylating with diazomethane. Two kinds of rubber sheets for medical use and 9 kinds of commercial rubber footwears (2 kinds of rubber boots and 7 kinds of shoes with rubber soles) were analyzed by the proposed method. Consequently, only 2-mercaptobenzothiazole (MBT) and/or dibenzothiazyl disulfide (MBTS) were found in all rubber samples. The ranges of contents were as follows : MBT, 21.7-1190 μg/g ; MBTS, 27.3-392 μg/g. Patch testing to MBTs was also studied in 40 patients with allergic contact dermatitis from rubber or poly vinyl chloride (PVC) materials (rubber group or PVC group), who were the same subjects in the previous papers. Generally speaking, rubber group reacted to MBTs in higher incidence than PVC group.
