摘要：The LC50 test method by use of Tubifex was developed for the evaluation of eco-toxicity of chemicals. The change of the observation period, the size of Tubifex or the test temperature affected little the LC50 value of 3, 5-dichlorophenol. Acute toxicity of 20 chemicals was tested at 20°C for 48 h by use of Tubifex of 30-50 mm in length. The test results were compared with the other three biological test methods ; EC50 of activated sludge respiration inhibition test, LC50 of Oryzias latipes (Himedaka ; red killifish) acute toxicity test and EC50 of Tetrahymena pyriformis proliferation inhibition test. A good correspondence was found in each two test method compared, and the regression analysis showed that the sensitivity of the Tubifex test lied between those of activated sludge and O. latipes tests.
