摘要:Celite 545 was applied to extraction of paraquat from human blood. The experimental procedure was as follows. Two ml of a sample blood adjusted pH 8.0 with 1 ml of Britton-Robinson buffer solution (pH 8.0) was injected into 5 g of celite 545 in centrifuge tube (50 ml), and mixed with glass rod. Then 30 ml of distilled water was put into the centrifuge tube, and mixed with glass rod. It was centrifuged at 3000 rpm for 3 min and the water layer was rejected. The remainder was washed three times more with 30 ml of distilled water. Five ml of 2 N sodium hydroxide solution and 5% sodium dithionate solution were added. After mixed, it was centrifuged at 3000 rpm for 3 min. The absorbance of the upper solution was measured at 600 nm by using glass cells. No detectable interference for the extraction of paraquat have been observed with other compunds, such as Ca (II), Cu (II), Fe (III), glycine, glucose, medazepam, barbital, and nereistoxin.