摘要：A head space method was studied for the quantitative determination of toluene in tissues by gas chromatography (GC). The method may be summarized as follows. Tissue was minced by extruding it from a plastic syringe into an equilibration vial containing water, sodium hydroxide, olive oil and an internal standard (ethylbenzene). As a standard sample, another equilibration vial having the same components plus authentic toluene but without tissue was used. Both vials were rapidly sealed with Teflon-rubber packing, and then shaken at 35°C for 2.5 h in a water bath. One milliliter of warmed head space gas was subjected to GC. The concentration of toluene in tissue was calculated by comparing T/I (T, peak height of toluene ; I, peak height of internal standard) of the tissue sample with that of the standard sample. This method was found to be useful for determining toluene in the range of 0.002 to 10 mg/g in tissue.
关键词：head space method;internal standard;GC;toluene tissue;olive oil;solubilization
