摘要：Microdetermination of manganese in red cells by flameless atomic absorption spectrometry (F. A. A. S.) was studied by use of rabbit blood. In a centrifuge tube (vol. 10 ml), 0.5 ml of red cells was digested with nitric acid and hydrogen peroxide on the boiling water bath. In order to avoid contamination from outside, a glass tube bended at right angles was put on the container and the wet digestion was carried out in the shielded room. The digested solution was dried on wet NaOH under reduced pressure in a desiccator. After dissolving the dried residue in 1.5 ml of 4 N HCl, iron was removed by twice extraction with 0.5 ml of tributyl phosphate. One ml of the aqueous phase and 0.5 ml of 0.01 % sulfosalicylic acid were put in another tube, and pH of the mixture was adjusted to about 10 with NH4OH indicated with phenolphthalein. After 0.1 ml of 5% hydroxylamine hydrochloride was added, manganese was extracted with 1 ml of 0.02 % dithizone-0.01 M ortho-phenanthroline chloroform soln. as ternary complex. Twenty μl of organic phase was submitted to measurement by F.A.A.S. Operating conditions were as follows : dry, 5A-20s ; ash, 70A-30s ; atomize, 220A-5s. Under these conditions, the calibration curve for standard manganese solutions was linear in the range of 0-50 ppb. Determination of manganese in red cells could be performed by absolute calibration method. Manganese contents in red cells of three rabbits were 18.1±0.7, 82.7±1.2 and 64.3±6.8 ppb, respectively.
关键词：manganese;flameless atomic absorption spectrometry;rabbit red cells;solvent extraction;wet digestion;ternary complex
