摘要：When washed cells of Kloeckera apiculata were suspended in n-butyl p-hydroxybenzoate solution, release of α-amino nitrogen from the cells into the medium was observed. There was also a release of thiamine into the medium on incubation of thiamine-accumulated cells with n-butyl p-hydroxybenzoate. A close correlation between the extent of leakage and the percentage of dead cells was found. The cell uptake of thiamine was inhibited at a concentration of n-butyl p-hydroxybenzoate that caused no release of α-amino nitrogen. Formation of protoplasts on incubation of the benzoate-treated cells with lytic enzyme was suppressed in comparison with intact cells. Protoplasts were lysed by n-butyl p-hydroxybenzoate on incubation of the protoplasts with the benzoate in 0.8M NaCl solution. In view of these results, it seems possible that n-butyl p-hydroxybenzoate attacks the cytoplasmic membrane of yeast.
