摘要：Experiments were designed to determine whether chelating agents would affect the amount of lead and of calcium, and alkaline phosphatase activity in the liver of rats treated with lead acetate. The rats were intraperitoneally given 200 mg/kg of sodium citrate and calcium disodium ethylenediaminetetraacetate (Na2Ca-EDTA) 6 days afer a single intraperitoneal administration of 200 mg lead/kg. The amount of liver calcium increased by administration of lead was reduced to the range of normal level after treatment with chelating agents, and lead accumulated after lead administration also decreased significantly. On the other hand, the hepatic alkaline phosphatase activities 6 days after lead administration decreased to about one-quarter compared with control rats. However, by the injection of sodium citrate or Na2Ca-EDTA to rats administered lead, the hepatic alkaline phosphatase activity recovered approximately to two-thirds and one-half of the control value, respectively. Thus, the removal of lead and calcium deposited in the liver by injection of chelating agents elevated hepatic alkaline phosphatase activity which was significantly inhibited after lead administration. Also, the data obtained in the present studies suggest that sodium citrate has an antagonistic effect on lead intoxication.
