摘要：The accumulation of metals in the several organs (hepato-pancreas, kidney, gills and spleen) of carp (Cyprinus carpio) was studied by two kinds of cadmium administration methods, namely, exposure to 5 mg Cd/l solution and intraperitoneal (i.p.) injection of 2 mg Cd/kg body wt. Cadmium concentrations in the kidneys accumulated by these two administration methods were highest among the organs, those in hepato-pancreas being next to the kidneys. Cadmium accumulation in hepato-pancreas and kidney was higher by the intraperitoneal injection than by the exposure. Zinc concentration was high in the spleen by the intraperitoneal injection, and that of copper was high in hepato-pancreas by the two administration methods. The cytosolic fractions obtained from carp hepato-pancreas, kidney, gills and spleen were further fractionated into high molecular fraction (HMF) and metallothionein (MT) fraction (MTF). The difference of concentrations of cadmium and zinc in the MTF and HMF by the two administration methods was studied. In the case of intraperitoneal injection, the cadmium concentration in the MTF was higher than that of the HMF in all organs. Compared with the control, the zinc concentration decreased in the MTF of hepatopancreas, but increased in that of gills by both administration methods. The induction of metallothionein in gills and spleen by the injection of cadmium was recognized, and two types of isometallothionein were separated (MT-I, MT-II). The metal composition in gills MT-I and spleen MT-I was mainly cadmium. The molar ration of cadmium, copper and zinc in gills MT-II was 48 : 2 : 1, and that of cadmium and zinc in spleen MT-II was 35.2 : 1.
