摘要：Carp (Cyprinus carpio) were injected intraperitoneally with 2 mg Cd/kg body weight as CdCl2 solution. Two types of isometallothioneins were separated from carp kidney by successive treatment with homogenization, ultracentrifugation, incubation, centrifugation, gel filtration and ion exchange chromatography. Purity was shown by polyacrylamide DISC-gel electrophoresis. The molecular weight of native metallothionein-I and -II was found to be 12000-13000. The disappearance of Cd-mercaptide bond was estimated under acidic conditions. Cysteic residues were about 30% in kidney isometallothioneins, Gly and Lys were high, but no aromatic amino acids or His residues were detected. In a double diffusion test, spur formation was shown between two hepato-pancreatic isometallothioneins, and also between hepato-pancreatic metallothionein-II and kidney metallothionein-II.
