摘要：In order to apply an aqueous toluene solution as standard-sample for the detection of toluene in the blood, the present study was carried out. The volatilities of some solvents in blood-sample were gas chromatographically measured by using head space gas method concerning the next problems : the kind of internal standard substance, warming temperature, the shaking effect for the volatilization of solvent, the kind of packing for enclosing head space gas in a vial and the diluting effect of blood with water. As the result, the following method was established. Ethylbenzene (chloroform or 1, 2-dichloroethane) as internal standard was added to vial having a five-fold diluted blood-sample or aqueous standard-sample. The vial was sealed with Teflon-rubber packing and shaken at 35°C for 20 min in water bath. One ml of the head space gas in a vial was subjected to gas chromatography. The concentration of toluene was calculated by comparing the result of blood-sample with that of standard-sample. This method permitted the use of aqueous standard solution and the measurement of toluene was possible over the range of 0.5 to 500 μg/ml in the blood.
关键词：head space gas method;internal standard;GC;toluene in blood
