摘要：A simple method for measurement of linoleic acid by high pressure liquid chromatography was developed to examine the degradation of linoleic acid in the presence of metals and their EDTA complexes. The chromatographic conditions were as follows : Column, Hitachi gel 3010-CH2OH (20cm×4mm) ; mobile phase, EtOH-0.01 N HCl (9 : 1) ; flow rate, 1 ml/min ; detector, UV (at 254nm). The degradation rate of linoleic acid was examined under following conditions : A mixture of 2.9ml of 0.05M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid) buffer (pH 7.2), 1.0 ml of 3 mM EDTA or distlled water, and 0.1 ml of 3 mM metal solution was placed in a test tube, 0.1 ml of 0.1 M linoleic acid in EtOH was added and the mixture was kept for 24 hr at 25°. A portion of the mixture (20 μl) was injected into the liquid chromatograph to measure residual linoleic acid. It was found that Mn2+, Cu2+, Fe3+ and Fe3+-EDTA remarkably accelerated the oxidative degradation of linoleic acid, while Mn2+-EDTA suppressed the degradation.
